The change of expression of c-fos and c-jun gene when using IN-1 alone or combination with NT-3 after spinal cord injury
10.3760/cma.j.issn.1008-1372.2011.01.003
- VernacularTitle:IN-1和NT-3联合作用对大鼠脊髓组织c-fos和c-jun基因表达变化的影响
- Author:
Ruisen ZHAN
;
Shuangxi SUN
;
Weiguo WANG
;
Shijie CHEN
- Publication Type:Journal Article
- Keywords:
Spinal cord injuries/DT;
Neurotrophin 3/AD;
Nerve growth factors/AI/AD;
Antibodies,monoclonal/AD;
Spinal cord/DE/ME;
Proto-oncogene proteins c-fos/ME;
Proto-oncogene proteins cjun/ME
- From:
Journal of Chinese Physician
2011;13(1):9-11,15
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the change of expression of c-fos and c-jun when using IN-1 alone or combination with NT-3 after spinal cord injury. Methods 120 adult health Sprague-Dawley (SD) rats were random divided into three groups, including control group, IN-1 group, and IN-1 combination with NT-3 group. All rats were killed at the scheduled time and its myeloid tissues were taken out. In each group, the expression of c-fos and c-jun gene was detected by using reverse transcription- polymerase chain reaction technique ( RT-PCR ). Result The transcriptional levels of c-fos decreased and c-jun increased when using IN-1 alone, and the levels changed more when using IN-1 combination with NT-3. The peak of c-fos reached to 0. 974 ±0. 126 in control group, 0. 834 ±0. 047 in IN-1 group, and 0. 698 ±0. 052 in IN-1 combination with NT-3 group, and the peak of c-jun reached 0. 642 ±0. 048, 0. 712 ±0. 050, and 0. 814 ±0. 041, respectively. Conclusion One of the mechanisms of IN-1 and NT-3 to protect the spinal cord might be through inhibiting the expression of c-fos and enhancing the expression of c-jun.
