Construction of DNA Vaccine pcDNA3.1(+)/Tetraspanin 2-A against Schistosoma japonicum and its Immuno-protective Effect in Mice
- VernacularTitle:日本血吸虫体表蛋白Tetraspanin 2-A核酸疫苗的构建及其对小鼠的免疫试验
- Author:
Peng ZHANG
;
Weina ZHANG
;
Cuiping REN
;
Miao LIU
;
Jijia SHEN
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Surface membrane protein;
DNA vaccine;
Protective immunity
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Tetraspanin 2-A(SjTsp2-A) gene was amplified by PCR.pcDNA3.1(+)/SjTsp2-A recombinant plasmids were constructed and transformed into E.coli DH5?.Twenty four BALB/c mice were randomly divided into pcDNA3.1(+) /SjTsp2-A group(A),pcDNA3.1(+)/SjGST group(B) and pcDNA3.1(+) group(C).Each mouse was injected through musculus quadriceps femoris by three times(two weeks interval) respectively with 100 ?g pcDNA3.1(+)/SjTsp2-A,pcDNA3.1(+)/SjGST,or pcDNA3.1(+).At two weeks after the final inoculation,mice were each challenged by 40?2 cercariae of S.japonicum.Forty-five days after infection,all mice were sacrificed,the number of worms collected and eggs in liver tisssue was counted.Anti-pcDNA3.1(+)/SjTsp2-A antibody was detected by ELISA and protein expression in quadriceps muscle by immunohistochemical staining.The worm reduction rate(44.4%) and egg reduction rate(28.4%) of group A was higher than those of group B and C(P
