Expression and biological function identification of TCR?9/?2-Fc protein in baculovirus vector expression system
- VernacularTitle:昆虫杆状病毒表达系统表达TCR?9/?2-Fc融合蛋白及其生物学功能的初步鉴定
- Author:
Yang GUO
;
Jing ZHENG
;
Yu HU
;
Lianxian CUI
;
Wei HE
- Publication Type:Journal Article
- Keywords:
TCR?9/?2-Fc;
baculovirus vector expression system
- From:
Basic & Clinical Medicine
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an expression system of TCR?9/?2-Fc protein by baculovirus vector expression system and identify biological function of expressed TCR?9/?2-Fc protein.Methods ?9Fc and ?2(OT3)Fc gene fragments were amplified by overlap PCR and inserted into expression vector pBACp10ph.The recombinant plasmid pBACp10ph-?9/?2(OT3)-Fc and the baculovirus DNA were co-transfected into sf9 cells.The expression position of TCR?9/?2(OT3)-Fc was identified by Western blot and the expression efficiency of ?9Fc and ?2(OT3)Fc was tested by flow cytometry(FCM).Furthermore,the binding activity of TCR?9/?2(OT3)-Fc protein with SKOV3 cells and MNS protein was evaluated with laser scanning confocal microslopy and surface plasmon resonance(SPR).Results The recombinant vector pBACp10ph-?9/?2(OT3)-Fc was constructed and TCR?9/?2(OT3)-Fc protein was expressed in sf9 cells.However,the expression efficiency of ?9Fc and ?2(OT3)Fc was quite different.It was proved that purified TCR?9/?2(OT3)-Fc protein can bind with SKOV3 cell and MNS protein.Conclusion TCR?9/?2-Fc protein is successfully expressed in baculovirus vector expression system and TCR?9/?2-Fc protein can simulate the binding activity of TCR in vitro.
