Detection of Different Genotyping of ?-Lactamases and Drug Resistance in Enterobater cloacae
- VernacularTitle:阴沟肠杆菌不同?-内酰胺酶基因型检测与耐药性研究
- Author:
Huayu GONG
;
Zhenguo LIU
;
Jiandang ZHOU
;
Limin CHEN
;
Haoye ZHANG
- Publication Type:Journal Article
- Keywords:
Enterobacter cloacae;
Three-dimensional extract test;
PCR;
?-Lactamases;
Resistance
- From:
Chinese Journal of Nosocomiology
2009;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the prevalence of ?-lactamases in nosocomial infections of Enterobacter cloacae,study the different genotyping of ?-lactamases,and analyze its independence in drug resistance. METHODS AmpC ?-lactamases and ESBLs were detected by three-dimensional extract tests.PCR was used to determine the genotype of ?-lactamases and their resistance to 13 kinds of antibiotics was detected by Kirby-Bauer method. RESULTS The sensitivity test showed that they were resistant to the most antibiotics.Among the 80 ESBLs strains,29 strains carried ESBLs gene,3 strains carried two different gene.Such as:TEM existed in 6,SHV-Ⅰ existed in 2,CTX-M-2 existed in 5,CTX-M-3 existed in 8 and CTX-M-9 existed in 11 strains.Twenty-six strains carried ampC.The resistant rate of SSBLs-producing E.cloacae to 12 antibiotics except merapenem was 66.7-100.0%.SSBLs strains of E.cloacae were higher than AmpC ?-lactamases in resistance to levofloxacin,cefepime,aztronem and trimethoprim sulpfamethoxazole with significant difference(P
