rAAV-CD151 activates PI3K/Akt/eNOS pathway and promotes neovascularization in swine
- VernacularTitle:CD151激活PI3K/Akt/eNOS通路并促进小型猪缺血心肌血管生成
- Author:
Houjuan ZUO
;
Sha WEN
;
Zhengxiang LIU
;
Tao LIU
;
Xiaochun LIU
;
Yu LIU
;
Jingyang LIN
;
Daowen WANG
- Publication Type:Journal Article
- Keywords:
CD151;
Myocardial infarction;
Angiogenesis
- From:
Journal of Medical Postgraduates
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The signal transduction pathway of phosphatidylinositol-3-kinase(PI3K) may play important roles in promoting angiogenesis induced by growth factors.The involvement of CD151,a member of transmember-4 superfamily,remains unclear.This study aimed to investigate the effects of the recombinant adeno-associated virus mediated CD151(rAAV-CD151) gene delivery on capillary density in myocardial infarction swine and its mechanisms.Methods: We established the swine model of myocardial infarction by coronary artery ligation,and intramuscularly injected rAAV-CD151,rAAV-GFP and rAAV-antiCD151 into the ischemic myocardium.Eight weeks after coronary artery ligation,we detected the expression of CD151,and measured capillary density by immunostaining for the von Willebrand factor.Results: Compared with the control and the rAAV-GFP groups,the rAAV-CD151 group showed a higher CD151 protein expression and capillary density in ischemic myocardium after myocardial infarction(P
