A new model-tracking of ?-cell development by generation of transgenic zebrafish
- VernacularTitle:新型的动物模型-可示踪胰岛?细胞发育的转基因斑马鱼
- Author:
Ming XIA
;
Xue PAN
;
Ming DENG
;
Yi JIN
;
Yi CHEN
;
Hesheng WANG
;
Deming KONG
- Publication Type:Journal Article
- Keywords:
zebrafish;
pancreatic ? cell;
transgenic;
insulin;
fluorescence protein
- From:
Basic & Clinical Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a pancreatic ?-cell developmet fish model with specific spatial expression patterns.Methods Molecular cloning,microinjection,whole embryo in-situ hybridization(WISH)and fluorescence microscopy in living were used to analyze of ?-cell development through generation of transgenic zebrafish.ResultsScreened and established pancreatic ? cells of transgenic zebrafish,and confirmed the fluorescence protein expression in the same spatiotemporal pattern with endogenous insulin gene to achieve dynamic monitoring islet ?-cell development situation in vivo.Conclusion The pancreatic ? cells of transgenic zebrafish animal model can successfully trace pancreatic ? cell development.
