Effects of A?_(25-35) on the expression of gene P21、CDK4、E2F1、BAX in PC12 cell
- VernacularTitle:A?_(25-35)对PC12细胞周期及P21、CDK4、E2F1、BAX基因表达的影响
- Author:
Zhaoyang XIE
;
Qifeng ZHU
;
Binhua WU
- Publication Type:Journal Article
- Keywords:
A?25-35;
PC12 cell;
cell cycle;
gene expression
- From:
Basic & Clinical Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of A?25-35 on the expression of gene P21,CDK4,E2F1 and BAX in cultured PC12 cells.MethodsPC12 cells were treated with 25 ?mol/L A?25-35,the relation between cell cycle redistribution and apoptosis was analyzed by flow cytometry(FCM).Protein and mRNA expression of P21,CDK4,E2F1 and BAX were detected by RT-PCR and Western-blot respectively.Results About 90% PC12 cells were found arrest on G0/G1 by FCM being deprived serum.Treated with 25 ?mol/L A?25-35 for 8,16,24 h,the percent of S phase cells was raised remarkably(P
