MAR increased transgene expression in stable transfected CHO cells
- VernacularTitle:MAR提高稳定转化的CHO细胞转基因表达
- Author:
Tianyun WANG
;
Zhongmin HAN
;
Xianjun YANG
;
Zhongsheng DONG
;
Qingyi WANG
- Publication Type:Journal Article
- Keywords:
matrix attachment region;
transgene;
gene engineering;
CHO cells
- From:
Basic & Clinical Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of human ?-globin matrix attachment region(MAR) on transgene expression in stably transfected CHO cells.Methods Expression vector was constructed,which contained the ?-globin MAR in both sides of Chloramphenicol acetyltransferase(CAT) reporter gene expression cassette in cis,then transfected into CHO cells.The CAT reporter gene expression was analyzed by ELISA method.Results The ?-globin MAR enhanced the CAT gene expression 5.5-fold in stably transformed CHO cells,while the transgene expression variation among individuals of transformants was decreased.Conclusion MAR increase transgene expression in stably transfected CHO cells.
