Effects of scallop skirt glycosaminoglycan on the expression of c-myc and TNF-? genes in proliferative vascular smooth muscle cells
- VernacularTitle:栉孔扇贝裙边糖胺聚糖对增殖的血管平滑肌细胞c-myc和TNF-? mRNA水平的影响
- Author:
Liying SHA
;
Ying YANG
;
Yi YIN
;
Shouyi DING
- Publication Type:Journal Article
- Keywords:
Chlamys farreri;
glycosaminoglycan(SS-GAG);
TNF-?;
atherosclerosis(AS);
in situ hybridization
- From:
Chinese Journal of Marine Drugs
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Chlamys farreri skirt glycosaminoglycan(SS-GAG) on the expressions of c-myc and TNF-? genes so as to explore the anti-atherosclerosis(AS) mechanism of SS-GAG.Methods The cell proliferation model of vascular smooth muscle cells(VSMC) was established by basic fibroblast growth factor(bFGF) induction.In Situ hybridization marked by non-isotope was applied to determine the effects of SS-GAG on the mRNA levels of c-myc and TNF in proliferative VSMC.Results The mRNA levels of c-myc and TNF in low dose group and high group of SS-GAG were obviously lower than that of model group(P
