Inhibitory effect of 4'-methylether-scutellarein on human choriocarcinoma JAR cells and the possible mechanism
- VernacularTitle:马鞭草C部位单体4′-甲醚-黄芩素对人绒毛膜癌细胞增殖的抑制作用
- Author:
Bo FENG
;
Changfen XU
- Publication Type:Journal Article
- Keywords:
4'-methylether-scutellarein(4-MS);
Choriocarcinoma JAR cell line;
Caspase 3;
Survivin;
p38 MAPK signal pathway
- From:
Chinese Journal of Cancer Biotherapy
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the inhibitory effect of 4'-methylether-scutellarein(4-MS),an extract from Verbena officinalis,on human choriocarcinoma JAR cell line and the possible mechanism.Methods:JAR cells were exposed to 4'-methylether-scutellarein of different concentrations for 48 h.MTT assay was used to examine the anti-proliferative effect of 4'-methylether-scutellarein.Flow cytometry was used to investigate the apoptosis and the changes of cell cycle.AO/EB double staining was applied to discriminate the apoptotic cells from dead ones.The changes of Survivin,p38-MAPK and Caspase 3 mRNA expressions were detected by RT-PCR in JAR cells treated with 4-MS.Furthermore,Western blotting assay was used to determine Survivin protein expression,phosphorylation level of p38 and Caspase 3 in JAR cells before and after 4-MS treatment.Results:4-MS inhibited the proliferation of JAR cells in a dose- and time-dependent manner(P
