High volume hemofiltration relieves acute lung injury induced by LPS in dogs
- VernacularTitle:高容量血液滤过减轻脂多糖诱导犬急性肺损伤
- Author:
Fen LIU
;
Zhenguo ZENG
;
Rong JIANG
;
Kejian QIAN
;
Nonghua LU
- Publication Type:Journal Article
- Keywords:
high volume hemofiltration;
LPS;
acute lung injury
- From:
Basic & Clinical Medicine
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the mechanism of High Volume Hemofiltration (HVHF) on acute lung injury (ALI) induced by LPS in dogs. Methods After injection of LPS (650 ?g/kg) via central vein within 30 min, Sixteen healthy hybrid male dogs were divided into control group and treatment group randomly (n=8). PaO2、PaCO2 in artery blood were recorded. Contents of TNF-?、IL-6 and IL-10 in plasm were measured by radioimmunity. The activity of NF-?B in lung homogenate was measured by flow cytometer. The content of surfactant protein B (SP-B) in lung homogenate was measured by Western-blotting.Changes of lung histopathology was observed via electron microscopy. Results After injection of LPS, PaO2 and PaO2/FiO2 began to decrease. PaO2 and PaO2/FiO2 in treatment group kept higher than that in control group (P
