Determination of human telomerase reverse transcriptase and telomerase activity in different tumor cell lines and its significance
- VernacularTitle:不同肿瘤细胞株人端粒酶逆转录酶及端粒酶活性的检测及其意义
- Author:
Xiaoxia YU
;
Yingai SHI
;
He DONG
;
Lihong ZHANG
;
Shan WU
- Publication Type:Journal Article
- Keywords:
telomerase;
cell line;
telomerase reverse transcriptase;
neoplasms
- From:
Journal of Jilin University(Medicine Edition)
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of humar telomerase reverse transcriptase (hTERT) and telomerase activity in HeLa,MCF-7,SMMC7721 PC-3m and U2OS cell lines. Methods The techniques of immunochemistry and TRAP-ELISA were employed to detect the expression of hTERT and telomerase activity in different tumor cell lines.Results The positive rate of hTERT in HeLa cells(93.75%?3.10%)was significantly higher than that in U2OS cells(2.75%?0.96%),besides the other three cell lines showed an positive rates of hTERT in MCF-7(92.50%?2.65%),SMMC7721 (53.75%?2.22%)and PC-3m(23.50%?2.89%); Meanwhile,the telomerase activity of HeLa cells (94.58%?3.49%) was also much higher than that of U2OS(3.02%?0.43%),likewise the telomerase activities of the other three cell lines (MCF-7,SMMC7721,PC-3m) were 73.90%?4.50%,66.67%?3.35% and 50.62%?1.96%, respectively. Conclusion The expression of hTERT and telomerase activity show obvious differences among five tumor cell lines,suggesting that telomerase inhibitors cannot effect on all the tumor cell lines.
