Effect of -1 SNP on CCNH promoter activity
- VernacularTitle:Cyclin H基因启动子区-1 SNP对转录活性的影响
- Author:
Peilin CHEN
;
Xin CHEN
;
Hongxia ZHENG
;
Qihan WU
- Publication Type:Journal Article
- Keywords:
CCNH gene;
SNP;
Dual-Luciferase Reporter assay system
- From:
Basic & Clinical Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of-1 site single nucleotide polymorphism(SNP) on CCNH gene promoter transcription activity.Methods PCR and site-directed mutagenesis technology were used to construct CCNH basic promoter and-1G mutate promoter.Dual-Luciferase Reporter assay system was used to detect the transcription activity of constructed promoter.Results In AD293 cells,the activity of-1G mutate type promoter was significantly lower than that of wild type-1T promoter(P
