In vitro isolation and culture of primary blood-brain barrier endothelial cells by double filtering technique
- VernacularTitle:两次过滤体外分离培养原代血脑屏障内皮细胞
- Author:
Jiexiao LIU
;
Chuanqiang PU
;
Yanlei HAO
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
96% 7-10 days after culture. Immunohistochemistry of Ⅷ factor polyclone antibody showed positive in cytomembrane and cytoplasm, but negative in cell nucleus. These were cells with blood-brain barrier. CONCLUSION: Ideal endothelial cells of blood-brain barrier can be cultured by the improved cultural method of double filtering.
