Diallyl trisulfide inhibites interleukin-1? expression induced by lipopolysaccharide through inhibiting NF-?B activation in murine lung
- VernacularTitle:二烯丙基三硫通过NF-?B抑制脂多糖诱导小鼠肺组织IL-1?表达
- Author:
Guijun ZHU
;
Shujin LI
;
Zhanbiao YU
;
Yuxiang ZHANG
;
Junfeng LIU
;
Zhenjie HU
- Publication Type:Journal Article
- Keywords:
diallyl trisulfide;
lipopolysaccharide;
acute lung injury;
cytokine;
nuclear factor-?B
- From:
Basic & Clinical Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of nuclear factor-kappa B(NF-?B)in the modulation of diallyl trisulfide(DATS)on interleukin-1?(IL-1?)expression induced by lipopolysaccharide(LPS)in mice with acute lung injury(ALI).Methods Mice were randomly divided into Control group,ALI group,DATS group,DATS prevention group and DATS treatment group.The expression of IL-1? mRNA in the lung tissue was detected by reverse transcription PCR(RT-PCR).NF-?B activity in the lung tissue was detected by electrophoresis mobility shift assay(EMSA).The expression of phospho-I?B and I?B were assayed by Western blot.Results The expression of IL-1? mRNA,NF-?B activity and the phospho-I?B expression in lung tissues increased significantly at ALI group(P
