A Modified Dye Test for Toxoplasma gondii Infection
- VernacularTitle:微孔板弓形虫染色试验
- Author:
Hui XU
;
Anping NI
;
Qingtao CUI
;
Ying HAO
- Publication Type:Journal Article
- Keywords:
Toxoplasma gondii;
Dye test;
Microplate
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
A modified dye test with microplate was to be established to detect Toxoplasma antibodies with cell-cultured Toxoplasma gondii. Numbers of stained and unstained tachyzoites were estimated in every 100 tachyzoites in each well after dyeing with methylene blue. The dilution with 50% tachyzoites stained was used as final dilution. Better results of the microplate dye test has been received when the concentration of tachyzoites in suspension reaches 109/ml with 1% sodium citrate as accessory factor.
