Effects of different concentrations of glucose on the expressions of osteoprotegerin,the ligand of osteoprotegerin and related cytokines in MG63 cells
- VernacularTitle:不同浓度葡萄糖对MG63细胞株护骨素和护骨素配体及其相关因子表达的影响
- Author:
Wei ZHOU
;
Qiuhe JI
;
Nanyan ZHANG
;
Jialin WANG
- Publication Type:Journal Article
- Keywords:
High glucose concentration;
MG63 cells;
Osteoprotegerin
- From:
Journal of Medical Postgraduates
2004;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the regulative effects of different concentrations of glucose on the expressions of osteoprotegerin(OPG),the ligand of osteoprotegerin(OPGL) and the related cytokines[tumor necrosis factor related apoptosis inducing ligand(TRAIL),macrophage colony-stimulating factor(M-CSF) and transforming growth factor ?(TGF-?)] in osteosarcoma MG63 cells.Methods:The expressions of OPG,OPGL,M-CSF,TRAIL and TGF-? mRNA was examined by reverse transcriptase(RT)-PCR.Results:High concentration glucose up-regulated the expression of OPGL,M-CSF and TRAIL but down-regulated OPG and TGF-? expression in the MG63 cells.Conclusion:One of the key pathogenetic factors of diabetic osteoporosis is that high concentration glucose leads to the down-regulated expression of OPG and TGF-? but the up-regulated expression of some bone-resorbing cytokines such as OPGL,M-CSF and TRAIL in osteoblasts,then stimulates osteoclast differentiation and activity,which potentiates bone resorption and bone loss.
