Quantification of IL-10 gene expression in induced sputum of asthmatic patients by competitive PCR
- VernacularTitle:竞争性逆转录-聚合酶链反应对哮喘患者诱导痰白细胞介素-10基因表达的定量分析
- Author:
Mengzhang HE
;
Jianhui LIANG
;
Jun XU
- Publication Type:Journal Article
- Keywords:
Competitive RT-PCR;
Interleukin-10;
Bronchial asthma
- From:
Journal of Medical Postgraduates
2004;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the role of anti-inflammatory cytokine IL-10 in the pathogenesis of asthma.Methods:IL-10 internal standards was constructed by restriction digest.The expression levels of IL-10 mRNA were determined in peripheral blood and induced sputum of 23 asthmatic patients by(RT-PCR) with this competitive internal standards. Results:There was no IL-10 mRNA expression in most of the asthmatics.IL-10 mRNA was detected in some catabatic patients.But IL-10 mRNA was readily detected in 29 normal subjects. Conclusion:Airway inflammation of asthma was related to the expression of IL-10 gene.Asthmatic patients may have difficulty in IL-10 synthesis with transcript.
