Expression of vascular basement membrane-derived multifunctional peptide in Pichia pastoris and identification of its bioactivity
- VernacularTitle:血管基膜衍生多功能肽的表达及其生物活性的鉴定
- Author:
Wenjian DAI
;
Man ZHANG
;
Shuping PENG
;
Jiangu CAO
- Publication Type:Journal Article
- Keywords:
vascular basement membrane-derived multifunctional peptide;
anti-tumor;
angiogenesis inhibitor;
vascular basement membrane;
tube structure formation of endothelial cell
- From:
Chinese Journal of Cancer Biotherapy
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To express vascular basement membrane-derived multiple peptide (VBMDMP) in Pichia pastoris and to identify its bioactivity. Methods: PCR technique was used to obtain the target fragment GST-VBMDMP, which was then cloned into pPIC9K vector. The resultant vector pPIC9K-GST-VBMDMP was transfected into Pichia pastoris cells with spheroplast and the positive recons was identified. The His + Muts transformant was shake-cultured in MGY at 30 ℃. The culture supernatant (1 ml) was collected for preservation at-70 ℃ every 24 hours while maintaining the concentration of methanol at 0.5% in the culture medium. SDS-PAGE analysis was used to detect the protein expression after 8 days and then animal and cell experiments were performed with GST-VBMDMP. Results: SDS-PAGE analysis found that protein express increased during 1-7 days and decreased after 8 days in MGY medium; GST-VBMDMP fused protein was obtained by Glutathione Sepharose 4B and it inhibited the artery endothelial cell tube structure formation in C57BL/6 mouse; GST-VBMDMP( 2, 6, 10 mg/kg)also significantly inhibited the primary cancer Lewis mouse (tumor inhibitor rates being 96.6%, 82.1%, and 61.2%, respectively)and metastatic lung tumors(tumor inhibitor rates being 96.8 %, 87.9 %, and 75.3%, respectively)(P
