Study of therapeutic adjuvant-free protein vaccine-HPV16Z-Hsp65-E6/E7
- VernacularTitle:HPV16Z-Hsp65-E6／E7无佐剂重组蛋白疫苗的研究
- Author:
Xiaobing WANG
;
Mo LI
;
Zhaoyang LIU
;
Haimei TIAN
;
Ping QU
;
Yanfen LI
;
Yi LIU
;
Dongyan CAO
;
Zhi LIANG
;
Dongwan CHENG
;
Changjun SHAO
;
Wei ZHANG
- Publication Type:Journal Article
- Keywords:
human papillomavirus 16;
therapeutic vaccine;
cervical cancer
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the biological effects of the HPV16Z-Hsp65-E6/E7 fusion protein vaccine on the tumor associated with HPV16 infection. Methods: We tested the cellular immune responsive intensity to the vaccine by the lymphocyte proliferation and CTL response, and studied the therapeutic effect of the vaccine on mouse TC-1 cell transplanted cancer in vivo and the influence on mouse lifetime. Results: The spleen lymphocytes from the C57BL/6 mouse immunized by the Hsp65-E6/E7 vaccine could proliferate obviously in the presence of the protein and TC-1 tumor cell could be lysed specifically by immune activated lymphocytes in vitro. This animal therapeutic experiment in vivo showed that the vaccine suppressed the growth of TC-1 cell transplanted tumor remarkably. Conclusion: The recombined vaccine can induce specific cellular immune response in vitro and suppress HPV16 positive TC-1 tumor cell growth obviously in vivo.
