Application of Autoradiography in Study of Cold Storaged Transplant Organs
- VernacularTitle:放射自显影在移植器官低温保存研究中的应用
- Author:
Xiaodong TAN
;
Yongfeng LIU
;
Yimin LI
;
Sanguang HE
- Publication Type:Journal Article
- From:
Journal of China Medical University
2001;30(2):101-102，107
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Our purpose was to study whether exogenous adenosine triphosphate (ATP) could enter liver cell or not. Methods: Using continuous hypothermic machine perfusion model of rat liver, we used autoradiography in this experiment. Results: The light microscope pictures of group A(no ［α-32P］ ATP in the perfusate) showed that there was no specially labeled silver pellet in or out of rat liver cell. But the light microscope pictures of group B (37 MBq ［α- 32P］ATP in the perfusate) showed that distribution of numerous ［α-32P］ATP autoradiographic silver pellets in rat liver cell, and no autoradiographic silver pellet was found in the hepatic sinus and vessel. Conclusion: Exogenous ATP could enter cold storaged rat liver cell.
