Studies on the Prokaryotic Expression Conditions of groEL from Endosymbionts in Bemisia tabaci
- VernacularTitle:烟粉虱内共生菌groEL基因的原核表达条件研究
- Author:
Zhoujin TAN
- Publication Type:Journal Article
- Keywords:
Bemisia tabaci,Endosymbiont, groEL,Prokaryotic expression,Physical and chemical factor
- From:
Microbiology
1992;0(04):-
- CountryChina
- Language:Chinese
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Abstract:
The study on prokaryotic expression conditions of groEL from endosymbiont in B. tabaci was conducted.The results showed that:The optimal IPTG content for inducing prokaryotic expression of groEL was 100?mol/L. When it was induced by IPTG at 35℃,higher GroEL product would be acquired.The optimal induced culturing time for prokaryotic expression of groEL was 4～5 hours.Higher inoculated quantity would benefit prokaryotic expression of groEL. A little NH~+_(4) content would improved prokaryotic expression of groEL, but prokaryotic expression of groEL would be inhibit by Ca~(2+)、Fe~(3+)、K~(+ )and Mg~(2+).When low content glucose was added to the LB medium,it would benefit prokaryotic expression of groEL.Prokaryotic expression of groEL would be inhibit by high content glucose.
