Sorting of oligodendrocytes expressing Nogo-A in membrane by flow cytometry
- VernacularTitle:流式细胞术分选在细胞膜表达Nogo-A的少突胶质细胞
- Author:
Nanxiang XIONG
;
Chi WANG
;
Hongyang ZHAO
;
Fangcheng ZHANG
;
Xianl ZHU
- Publication Type:Journal Article
- Keywords:
Nogo-A;
Oligodendrocyte;
Flow cytometry;
Expression
- From:
Journal of Medical Postgraduates
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the expression of Nogo-A in membrane of mature oligodendrocyte, and obtain these living cells. Methods:Mature oligodendrocytes were stained by indirect immuno-fluorescent technique.Stained unpermeable oligodendrocytes were sorted by flow cytometry. Results: The percentage of mature oligodendrocytes with Nogo-A expression in membrane was 3.36%. Conclusion:Nogo-A could be expressed in membrane of mature oligodendrocytes,and the ratio of positive cells was about 3.36%.
