Protective effect of polypeptide from Chlamys farreri on UVB-irradiated murine thymocytes in vitro
- VernacularTitle:扇贝多肽对UVB辐射小鼠胸腺淋巴细胞的保护作用
- Author:
Haiying CHEN
;
Songmei ZHAN
;
Xiao CHU
;
Meizhi WANG
;
Yuejun WANG
;
Ruiyao CUI
;
Chunb WANG
- Publication Type:Journal Article
- Keywords:
Polypeptide from Chlamys farreri (PCF);
UVB;
radioprotection;
thymocytes;
mice;
apoptosis
- From:
Chinese Journal of Marine Drugs
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the radioprotective effect of polypeptide from Chlamys farreri (PCF) on ultraviolet B(UVB)-irradiated murine thmocytes in vitro. Methods The murine thymocytes were exposed to UVB radiation. MTT method was used to detect the cell viability. The activities of intracellular glutathione peroxidase (GSH-Px), superoxided dismutase(SOD) and catalase (CAT) were measured. Intracellular reactive oxygen species (ROS) and the effect of PCF on UVB-induced apoptosis were investigated by flow cytometry. Results PCF could greatly enhance the viability of murine thymocyte and markedly promote the activities of GSH-Px, SOD and CAT, while the amount of ROS was decreased. PCF could inhibit UVB-induced thymocyte apoptosis. Conclusion PCF has significant radioprotective effects on UVB-irradiated murine thymocytes in vitro.
