Transfection of human endostatin gene in umbilical cord CD34~+ hematopoietic stem cell
- VernacularTitle:血管内皮抑素基因转染人脐带血CD34~+造血干细胞的实验研究
- Author:
Jifeng FENG
;
Liangjun ZHU
;
Ji HU
- Publication Type:Journal Article
- Keywords:
Gene transfer;
Umbilical cord blood;
Hematopoietic stem cells;
Endostatin
- From:
Journal of Medical Postgraduates
2004;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To transfer human endostatin gene into umbilical cord CD34 + hematopoietic stem cells and detect its expression and excretion. Methods: Human endostatin gene was transferred into human umbilical cord CD34 + hematopoietic stem cells by retroviral pLNCX to build endostatin-transferred cell line.RT-PCR and Western blot analysis were applied to examine the transfection and expression of endostatin gene. Results:RT-PCR proved that genome of endostatin-transferred CD34 + hematopoietic stem cells contained a 550 bp fragment of human endostatin .The expression and excretion of human endostatin from endstatin-transferred hematopoietic stem cells were confirmed by Western blot analysis. Conclusion:Human endostatin gene can be transferred into CD34 + hematopoietic stem cells.
