Preparation of anti-Rh(D) reagent serum by using Rh(D) positive erythrocyte absorption
- VernacularTitle:用Rh(D)阳性红细胞吸收制备抗-D试剂血清
- Author:
Ruiqing XIAO
;
Wucun LIN
;
Jie ZENG
- Publication Type:Journal Article
- Keywords:
Rh anti-D reagent serum;
Absorption test;
Erythrocyte with positive Rh(D)
- From:
Chinese Journal of Blood Transfusion
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a new method of preparing anti-Rh(D) reagent serum in order to help overcome the shortage of Rh(D) negative red blood cells(RBCs). Methods Anti-Rh(D) containing type A and B sera were mixed. Through dilution and neutralization, the titer of complete antibodies decreased to 2. Anti-Rh(D) saturated Rh(D)positive RBCs were prepared by adding anti-Rh(D) containing serum to Rh(D) positive RBCs so that all the Rh factors on the cells were saturated. The anti-Rh(D) saturated type A and type B RBCs were added to the mixed plasma to absorb the remaining anti-A and anti-B. Results The acquired regent sera had a high anti-Rh(D) titer with a high specificity. Conclusion Anti-Rh(D) saturated Rh(D) positive RBCs absorption after neutralization can help with the production of human origin anti-Rh(D) reagent serum and save a large amount of Rh(D) negative evythvocytes.
