Research on human peripheral blood dendritic cell Inducing Th1/Th2 differentiation
- VernacularTitle:人外周血树突状细胞诱导Th1/Th2细胞分化的研究
- Author:
Guolin YUAN
;
Ping ZOU
;
Zichao CHEN
- Publication Type:Journal Article
- Keywords:
Dendritic cell;
Th0;
Th1;
Th2
- From:
Journal of Medical Postgraduates
2004;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study human peripheral blood dendritic cells(DC) inducing Th0 differentiation to Th1/Th2 at different DC/Th0 ration。 Methods: Mononuclear cells in healthy human peripheral blood were induced to DC through tradition method,4 days of culture of mixed DC/Th0 different ration( 1∶4 and 1∶300),harvesting Th,after 48 h of CD3 mAb and CD28 mAb expantion,in supernant IL-4 and IFN-? measurement were determined using ELASA。 Results:Expresse CD83(73.4%), CD86 (90.3%), HLA-DR(68.5%),CD40(73.7%).DC/Th0 ration(1∶4) produced IFN-? (34?4.3) ?g/L, a little amount of IL- 4(25?4.3) ng/L. DC/Th0 ration(1∶300) produced IFN-?(3.5?1.2) ?g/L, a large amount of IL- 4(350?120) ng/L. Conclusion:Highly mixed DC/Th0 ration induced Th0 differentiation to Th1, low mixed DC/Th0 ration induced Th0 differentiation to Th2, DC regulating immunology response type were plastic which provided the basis for clinic GVHD therapy.
