Use of human papilloma virus type 6bL_1 virus-like particles to detect sera antibodies in patients with condylomata acuminata
- VernacularTitle:用人乳头瘤病毒6bL_1病毒样颗粒检测尖锐湿疣血清抗体
- Author:
Kedong XIA
;
Lifang ZHANG
;
Shao CHEN
;
Lianlian CAI
;
Feiyun ZHENG
- Publication Type:Journal Article
- Keywords:
Papillomavirus;
Virus-like particle;
Condylomata acuminata;
Antibodies
- From:
Chinese Journal of Laboratory Medicine
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the serum antibody to HPV6 in patients with condylomata acuminata (CA) by use of human papilloma virus(HPV)6bL 1 virus-like particles (VLPS), and to assess the significance of detecting HPV infection. Methods HPV6bL 1 VLPS were produced by a HPV6bL 1 recombinant baculovirus in insect cells,purified by CsCl density gradients centrifugation,and used for ELISA to screen 136 human serum samples in three groups. Results The prevalence of antibody to HPV6bL 1 VLPs was much higher in patients with codylomata acuminata (75%Ab+;mean abs 0.111?0.094) than that in patients with cervical cancer (14.3%Ab+;mean Abs 0.029?0.022)and that in control groups (2.9%Ab+;mean Abs 0.012?0.024). The differences among three groups are significant(P
