Introduction of Rb, p53, p16 and H-ras Antisense UNA Suppresses Tumorigenicity in Human Gastric Cancer Cell Lines
- VernacularTitle:导入Rb、p53、p16和H-ras反义RNA对人胃癌细胞恶性增殖的影响
- Author:
Wenmei LI
;
Youyong LU
- Publication Type:Journal Article
- Keywords:
multiple gene alterations;
gastric cancer;
tumorigenesis
- From:
Chinese Journal of Cancer Biotherapy
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Our previous investigation have demonstrated that multiple genes alterations, such as deletion of Rb, p53 and p16 gene, point mutation of H-ras gene were detected in cell line and solid tumor of human gastric cancer. We have transfect-ed the independent construct containing Rb, p53, p16 and expression of H-ras antisense RNA respectively into human gastric cancer cell line, and we have analyzed the biological properties of several independent transfectant cell lines, which express exogenous Rb, p53, p16 and H-ras antisense RNA respectively. The cell growth ability was inhibited by introduction of p53 and H-ras antisense RNA, and tumorigenicity also suppressed significantly by p53, p16 and H-ras antisense RNA. These results indicated that alterations of p53, p16 and H-ras gene were involved in human gastric car-cinogenesis.
