Expression of Exogenous r-Interferon Genes in Human HepatoceUuIar Carcinoma Cells Following Retroviral Transduction
- VernacularTitle:逆转录病毒载体介导的?-干扰素基因在人肝癌细胞中的表达研究
- Author:
Shubing QIAN
;
Tengfei ZHANG
;
Liang HU
- Publication Type:Journal Article
- Keywords:
retroviral vector;
7-Inteferon;
hepatocellular carcinoma;
HIA class I molecule
- From:
Chinese Journal of Cancer Biotherapy
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Retroviral vector pLXSN was employed to introduce human r-Interferon (IFN-?) gene into four human hepa-tocellular carcinoma cell lines (HCC). The G418-resistant colonies were isolated and cloned. PCR and RT-PCR analysis indicated that the integration and expression of IFN-? gene was shown only in the transduced cells. Using a bioassay method, we found that all genetically modified HCC cells can secrete varied amount of IFN-?. The results of flow cytome-try showed that the cell surface expression of HLA class I molecules significandy increased following transduction. Moreover , we firsdy indicated that the increase in the expression of one specific HLA class I antigen, HLA-A2, was almost in the same magnitude as that of the total HLA class I molecules after transduction with IFN-r gene.
