In Vitro Study of the Effects of Interferon-? on Chronic Myelogenous Leukemia Cells in LTBMC
- VernacularTitle:干扰素-?对LTBMC中慢性粒细胞白血病细胞作用的体外研究
- Author:
Chengwen LI
;
Lugui QIU
;
Wenwei YAN
- Publication Type:Journal Article
- Keywords:
leukemia;
IFN-(?)a;
LTBMC
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The effects of interferon-? (IFN-?) on chronic myelogenous leukemia cells were studied in vitro by long-term bone marrow culture(LTBMC). There were no inhibition of cellularity from non-adherent layers and formation of adherent layers, however, CFU-GM from non-adherent layers was inhibited at IFN-? 103U/ml and 104 U/ml groups, when IFN-?was added only at initiation of culture. If IFN-? was continuously added weekly, the cellularity and CFU-GM of non-adherent layers were significantly inhibited, and the formation of adherent layers was inversely associated with the increasing concentration of IFN-?. in addition, Ph(+) cells in non-adherent layers were disappeared early and the percentage of Ph(-) population was increased with the combination of IFN-? and LTBMC. It is concluded that IFN-?selectively inhibits the later CFU-GM of CML cells and the development of stromal cells, the combination of IFN-?and LTBMC might exert a synergically purging effect on Ph(+) CML cells
