Effects of Electroacupuncture plus Oxygenmedicine on the Expression of Bcl-2 and Bax Proteins in the Hippocampal CA 1 Area in Rats with Global Cerebral Ischemia/Reperfusion Injury
- VernacularTitle:电针药氧对大鼠全脑缺血再灌注后Bcl-2、Bax蛋白表达的影响
- Author:
Yuan BU
;
Peiying ZHANG
;
Deqin GENG
;
Jinxia CAO
;
Jizhen LI
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia/reperfusion;
Electroacupuncture;
Oxygenmedicine;
Hippocampal CA 1 area;
Bcl-2;
Bax
- From:
Acupuncture Research
2010;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of electroacupuncture (EA) plus oxygenmedicine (OM) on the expression of Bcl-2 and Bax in the hippocampal CA 1 area in cerebral ischemia/reperfusion injury (CI/RI) rats. Methods Thirty SD rats were randomized into sham-operation,model,EA,OM,EA+OM groups (n=6/group). CI/RI model was established by using modified Pulsinelli 4 vessel occlusion and reperfusion. EA (100 Hz,3.5 mA) was applied to "Baihui" (GV 20) and "Zusanli" (ST 36) 30 min,once daily for 4 days. Rats of OM and EA+OM groups were put into a box filled with oxygen and atomized herbal medicines containing Bingpian (Borneolum),Shexiang (Moschus),Huangjing (Rhizoma Polygonati),Shouwu (Radix Polygoni Multiflori),etc. for 30 min,once daily for 4 days. Bcl-2 and Bax expression of the hippocampal CA 1 area was detected by immunohistochemistry. Results Compared with sham group,the numbers of Bcl-2 immunoreaction (IR) and Bax IR positive cells,and the immunoactivity of Bcl-2 IR and Bax IR positive products in the hippocampal CA 1 area were increased significantly in model group (P0.05). Conclusion EA and OM and EA+OM can effectively regulate the expression of Bcl-2 and Bax in the hippocampal CA 1 area in CI/RI rats,and the effects of EA+OM are significantly superior to those of simple EA and simple OM,which may contribute to their effect in improving cerebral ischemia.
