Isolation and purification of a lectin from roots of Astragalus membranaceus
- VernacularTitle:一种膜荚黄芪凝集素的分离纯化研究
- Author:
Lifen ZHU
;
Qiaojuan YAN
;
Zhengqiang JIANG
;
Narasimha KUMAR
;
Linhua HUANG
- Publication Type:Journal Article
- Keywords:
Astragalus membranaceus(Fisch.)Bunge;
lectin;
purification;
affinity chromatography
- From:
Chinese Traditional and Herbal Drugs
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate and purify a lectin from the roots of Astragalus membranaceus.Methods The protein was purified using a combination of 20%—60% ammonium sulfate fraction and ConA-Sepharose 4B affinity chromatography.Results The purified protein appeared as a single band with molecular mass of 3.15?104 on SDS-PAGE and the relative molecular mass was estimated by gel filtration on a calibrated Superdex 75 column with apparent molecular weight of 3.35?104.This lectin was a glycoprotein with a neutral carbohydrate content of 10.7%.Conclusion A lectin is isolated and purified from the roots of A.membranaceus for the first time.It is a monomer glycoprotein and its specific activity is 391.9 U/mg.
