Determination of Paeoniflorin in Dahuang Zhechong Tablets by HPLC
- VernacularTitle:HPLC法测定大黄虫片中芍药苷的含量
- Author:
Guoqiong LUO
;
Bin HUANG
;
Rongzhen JIANG
- Publication Type:Journal Article
- Keywords:
Dahuang Zhechong Tablets;
paeoniflorin;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for determining the content of paeoniflorin in Dahuang Zhechong Tablets. Methods Stationary phase was C18 column (4.6 mm?250 mm, 5 ?m), mobile phase was acetonitrice-0.1% phosphoric (14∶86), detection wavelength was 230 nm, flow rate was 1.0 mL/min and column temperature was 30 ℃. Results There was good linear relationship between the concentration of paeoniflorin and area integral value in the range of 0.10～0.90 ?g, r =0.999 7. The average recovery was 99.98% with RSD=0.81% (n =6). Conclusion This method is simple, accurate, with good reproducibility and high precision, and can be used to control the quality of Dahuang Zhechong Tablets effectively.
