Counteraction of Ganoderma Lucidum Polysaccharide on the Inhibition of Mice Splenocyte Proliferation and IL-1? and IL-2 mRNA Expression Induced by Prostaglandin E2
- VernacularTitle:灵芝多糖对前列腺素E2抑制小鼠脾细胞增殖及IL-1?,IL-2 mRNA表达的拮抗作用
- Author:
Qun ZHANG
;
Linsheng LEI
;
Chuanlin YU
;
Shuguang WU
- Publication Type:Journal Article
- Keywords:
GANODERMA LUCIDUM POLYSACCHARIDE/pharmacology;
NEOPLASMS /TCD therapy;
NEOPLASMS/immunology;
CELL CULTURE
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2004;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the counteraction of Ganoderma lucidum polysaccharide(GLP) on the inhibition of mice splenocyte proliferation and IL-1? and IL-2 mRNA expression induced by prostaglandin E2(PGE2).Methods Mixed lymphocyte culture reaction(MLR) method was used for the experiment.The lymphocyte proliferation was determined by MTT method,and the levels of IL-1? and IL-2 mRNA expression were evaluated by semi-quantitative reverse transcriptase polymerase chain reaction(RT-PCR).Results After co-culture with PGE2 for 48 hours,the splenocyte proliferation was inhibited to some extent,and the difference was significant when the concentration of PGE2 was over 10?mol/L(P
