Determination of anthraquinone aglycones of Radix et Rhizome Rhei in Ganning Granule by HPLC
- VernacularTitle:肝宁颗粒中大黄所含5种蒽醌苷元的HPLC测定
- Author:
Shumei YUE
;
Lingling FENG
;
Xiaoxiao QI
;
Shaohui WAN
- Publication Type:Journal Article
- Keywords:
Ganning Granula;
rhubab;
HPLC;
anthraquinone aglycones
- From:
Chinese Traditional Patent Medicine
1992;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To determine Radix et Rhizoma Rhici in Ganning Granula(Radix et Rhizoma Rhei,Rhizoma Chuanxiong). METHODS: In order to determine five anthraquinone aglycones of Radix et Rhizoma Rhei,the HPLC system consisted of Nucleosil ODS column,methand-0.1% phosphoric acid(85∶15) mixture as mobile phase,with detection wavelength at 430 nm. RESULTS: HPLC showed that the linearity ranges were as follows: aloe-emodin concentration was at the range of 0.041 6-0.291 2 ?g,the rhein concentration was at the range of 0.037 1-0.259 8 ?g,the emodin concentration was at the range of 0.045 8-0.320 3 ?g,the chrysophanol concentration was at the range of 0.049 60.347 2 ?g,the physcion concentration was at the range of 0.020 6-0.144 5 ?g.The average recoveries of five anthraquinone aglycones were between 96.6%-98.4%.CONCLUSION: The method is simple,accurate,sensible and with good repeatability and can be used for quality control of Ganning Granule.
