The antiviral effect of interleukin 29 against hepatitis B virus in vitro
- VernacularTitle:白介素29体外抗乙肝病毒作用
- Author:
Yu CHAI
;
Xin LUO
;
Hailiang HUANG
;
Daojun HU
;
Zize TAO
;
Shengquan ZHANG
- Publication Type:Journal Article
- Keywords:
IL-29;
hepatitis B virus;
HepG2.2.15 cell
- From:
Chinese Journal of Biochemical Pharmaceutics
2009;30(6):368-370,374
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To explore the antiviral effect of interleukin 29(IL-29) on hepatitis B virus in vitro.Methods To study the antiviral effect of IL-29 against hepatitis B virus by the amount of HBV mRNA detected .Through the quantity of mRNA translated from genes of MxA,2′,5′-OAS,PKR and RNase L as well as the signal pathway induced by IL-29,we used RT-PCR and Western blot to discuss the anti-hepatitis B virus mechanism which was stimulated by IL-29.Results The amount of HBV mRNA in HepG2.2.15 cells was reduced by stimulation of IL-29.The expression of MxA and 2′,5′-OAS was up-regulated,as well as P-ERK and P-AKT were activated by IL-29.Conclusion These findings showed that IL-29 had obvious antiviral activity towards HBV in HepG2.2.15 cells.
