Simultaneous determination of camphor and menthol in Compound Dexamethasone Acetate Cream by GC
- VernacularTitle:气相色谱法同时测定复方醋酸地塞米松乳膏中樟脑和薄荷脑的含量
- Author:
Jingguan WANG
;
Yinghua FU
;
Lingxian ZHU
- Publication Type:Journal Article
- Keywords:
camphor;
menthol;
Compound Dexamethasone Acetate Cream;
GC
- From:
Chinese Traditional Patent Medicine
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish the capillary GC method for separating and determining both camphor and menthol in Compound Dexamethasone Acetate Cream. METHODS: The methyl salicylate was used as internal substance and the sample solution was prepared by extracting with anhydrous ethanol at(60 ?C.) The GC test conditions consisted of Agilent HP-INNOWAX(30 m?0.320 mm?0.25 ?m) as the stationary phase,nitrogen as the carrier gas,split ratio of 25.0(∶)1.The flow rate was 1.5 mL/min,column temperature was at 145?C,inlet temperature was(200 ?C),FID detection was(250 ?C),injection volume was 0.6 ?L. RESULTS: The linearities of camphor and menthol were good,in the range of 0.303-3.030 g/L with r=0.999 9 for camphor and in the range (0.306)-(3.060) g/L with r=0.999 9 for menthol.The average recoveries of high,middle and low three concentrations for menthol were 100.6%,100.2%, 99.7%;RSD was 0.58%,0.65%,0.88% and for camphor were 100.3%,(99.8%),(97.8%);RSD was 0.43%,0.78%,1.0%. CONCLUSION: This method is convenient,rapid,accurate and reproducible,and can be used for quality control of Compound Dexamethasone Acetate Cream.
