Chemistry fingerprints of water-soluble constituents of Salvia miltiorrhizae Bge.by HPLC
- VernacularTitle:丹参药材水溶性成分HPLC化学指纹图谱研究
- Author:
Xuli TANG
;
Jing LIU
;
Guoqiang LI
- Publication Type:Journal Article
- Keywords:
Salvia miltiorrhizae Bge.;
fingerprint;
RP-HPLC;
SPSS
- From:
Chinese Traditional Patent Medicine
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish the HPLC-fingerprint of the water-soluble constituents of Salvia miltiorrhizae Bge.on 18 batches from 9 areas in China. METHODS: Samples of Salvia miltiorrhizae Bge.from different areas were determined by Agilent 1100 DAD-HPLC on Sino Chrom ODS-BP column(250 mm?4.6 mm,5 ?m),gradient elution with methanol-water(0.7%H_3PO_4) as mobile phase,column temperature was at 30℃,flow rate was 1 mL/min,wavelength was at 280 nm,and the inject volume was 20 ?L. RESULTS: The HPLC-fingerprints of the water-soluble constituents from Salvia miltiorrhizae Bge.,including 12 mutual peaks,were developed after determination of 10 batches of drugs according to SPSS analysis.,and in which,3 characteristic components were recognized. CONCLUSION: The separation effect of peaks in fingerprints is better and the characteristic is also obvious.The RSD values of precision and reproducibility are less than 5%.This method can be used for the quality control of Salvia miltiorrhizae Bge.
