Analysis of Dual-wavelength Fingerprint Chromatogram of Buzhong Yiqi Decoction
- VernacularTitle:补中益气汤双波长融合指纹图谱的整体性分析
- Author:
Rufeng HUA
;
Keer HUANG
;
Xuehong KE
;
Jinfu CHEN
- Publication Type:Journal Article
- Keywords:
BuzhongYiqi Decoction;
Combination of fingerprint chromatogram;
HPLC-PDA;
Quality control
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the fingerprint chromatogram of Buzhong Yiqi Decoction(BYD) by multiple wavelength method,and to analyze the substance of formulas as a whole.Methods HPLC-PDA method was adopted.The chromatographic conditions were as follows:Hypersil ODS2 analytical column,the mobile phase consisting of 0.05 %phosphate acid and acetonitrile with gradient elution,detecting wavelength at 254 and 280 nm,the flow rate being 1.0 mL/min and the temperature of the column at 30 ℃.Results The methodological results were good.The results of dual-wavelength fingerprint chromatogram showed the all-around information of the fingerprints at 254 and 280 nm.The similarity of 10 batches of BuzhongYiqi Decoction was over 0.98.Conclusion Dual-wavelength fingerprint chromatogram can realize the analysis of a formulas as a whole,which supplies references for improving the quality control of BuzhongYiqi Decoction.
