?-Synuclein phosphorylation takes part in mouse dopaminergic neuron protection
- VernacularTitle:?-突触核蛋白磷酸化参与小鼠多巴胺能神经元的保护作用
- Author:
Qi LIU
;
Chunli DUAN
;
Bo WU
;
Chunxiang FAN
;
Tao ZHANG
;
Huanying ZHAO
;
Chunli ZHAO
;
Hui YANG
- Publication Type:Journal Article
- Keywords:
?-synuclein;
phosphorylated ?-synuclein;
neuron protection;
dopaminergic cells
- From:
Basic & Clinical Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of phosphorylation at Serine 129 in regulating the neurotoxicity of ?-synuclein. Methods Wild type and phosphomimic mutant ?-synuclein genes were over-expressed in mouse dopaminergic cells MN9D using retrovirus. The cell viability was examined using CCK-8 assay and cell morphology was observed by immunofluorescence microscopy. Results The result of real time PCR showed that WT/?-SYN and S129D/?-SYN genes were overexpressed in MN9D as compared to uninfected MN9D and vector control group(P
