Determination of Ferulic Acid in Callus of Ligusticum Chuanxiong by HPLC
- VernacularTitle:RP-HPLC法测定川芎愈伤组织中阿魏酸的含量
- Author:
Beilei ZHANG
;
Xingxing XIA
;
Qin CHEN
- Publication Type:Journal Article
- Keywords:
Ligusticum chuanxiong;
callus;
Ferulic acid;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the determination of Ferulic acid in callus of Ligusticum chuanxiong. Methods The separation was performed on ZORBAX 300SB-C18 (250 mm?4.6 mm, 5 ?m) column, using acetonitrile-1% triethylamine (15∶85) as mobile phase, detected at 320 nm. The flow rate was 1.0 mL/min, the column temperature was 27 ℃. Results The linear range of Ferulic acid was 0.93～4.67 ?g/mL, r =0.999 8, the average recovery was 99.98% and RSD was 1.63%. Conclusion The method was rapid, simple, accurate and stability, and can be used for the quantitiative determination of Ferulic acid in Ligusticum chuanxiong callus.
