Study of cell-mediated immune response induced by HPV DNA vaccine in combination with IL-15 expression plasmid
- VernacularTitle:IL-15表达质粒联合HPV基因疫苗诱导细胞免疫应答的实验研究
- Author:
Xin SUN
;
Shunqiang GAO
- Publication Type:Journal Article
- Keywords:
Interleukin-15;
Adjuvant;
Human papillomavirus;
DNA vaccine
- From:
Chinese Journal of AIDS & STD
2007;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of IL-15 eukaryotic expression plasmid on the Ag-specific immune responses induced by HPV 16 E7 vaccine in mice.Methods An eukaryotic expression plasmid encoding IL-15 gene coding region was constructed and named as pcDNA3.1IL-15.Female BALB/c mice were injected intramuscularly with pcDNA3.1-IL-15 and HPV16 E7 gene vaccine.After the immunization,the concentration of serum IFN-? were tested.Single-cell suspensions of splenocytes were prepared from mice and were re-stimulated with HPV16 E7 protein.Then,the T cell proliferation assay was measured by the MTT method.Results The concentration of serum IFN-? in mice after co-injection with pcDNA3.1-IL-15 and pcDNA3.1-E7 was raised to 414.1pg/ml,which was significantly higher than that of mice co-injected with pcDNA3.1 and pcDNA3.1-E7 or mice injected with pcDNA3.1-E7 only(P
