FUNCTION OF p21-ACTIVATED KINASE 2 IN XENOPUS OOCYTE CYTOKINESIS
- VernacularTitle:p21活化激酶2在爪蟾卵母细胞的细胞质分裂过程中的作用
- Author:
Daye CHENG
- Publication Type:Journal Article
- Keywords:
p21-activated kinase;
Oocyte;
Cytokinesis;
Microinjection;
Laser scanning confocal microscopy;
Xenopus
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
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Abstract:
Objective Study the function of p21-activated kinase 2 in Xenopus oocyte cytokinesis. Methods Xenopus oocyte was used as a model,and MPF activity and PAK2 phosphorylation status were observed during oocyte maturation.The specific inhibitor of PAK2 activity,PAK2-NT(PAK2-N-terminal) was used to microinject into Xenopus oocyte.Under fluorescent microscope germinal vesicle breakdown was observed during cytokinesis.To further observe the changes of F-actin and spindle,laser scanning confocal microscopy with time-lapse method was employed. Results Occurrences of germinal vesicle breakdown in control oocytes was similar to those oocytes injected with PAK2-NT mRNA,but no cytokinesis was observed in oocytes injected with PAK2-NT mRNA.Conclusion These results indicated that PAK2 might involved in Xenopus oocytes cytokinesis.
