EXPRESSION OF POSTSYNAPTIC DENSITY PROTEIN-93 IN INJURIED SPINAL CORD OF RAT
- VernacularTitle:突触后密度蛋白-93在大鼠脊髓损伤后的表达变化
- Author:
Shangfeng GAO
- Publication Type:Journal Article
- Keywords:
PSD-93;
Spinal cord injury;
nNOS;
Real-time PCR;
Western blotting;
Immunofluorescence;
Rat
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression and distribution of postsynaptic density protein-93(PSD-93) after spinal cord injury(SCI). Methods With improved Allen's method,acute rats SCI models were established.Real-time PCR and Western blotting were used to detect the changes of PSD-93 mRNA and protein expression after SCI.The changes of PSD-93 localization after injury were investigated by immunofluorescence double staining. Results The expression of PSD-93 mRNA had a gradually decrease trend after SCI.The minimal mRNA was present at 3days;whereas the protein levels up-regulated significantly and peaked at 1day after injury.Immunofluorescence double staining indictated that PSD-93 was highly expressed on the activated microglias and astrocytes except that it co-localized with nNOS in the impaired spinal cord.Conclusion The time course of PSD-93 and its mRNA is vary significantly after SCI,and colocalized with nNOS and activated glias.These results indicate that PSD-93 may participate in the following response after SCI.
