Quantitative determination of ophiopogonin D and ophiopogonin D′ in Fibre Ophiopogon enriched extract by macroporous resin
- VernacularTitle:HPLC-ELSD测定川麦冬须根大孔树脂富集物中麦冬皂苷D、D′的含量
- Author:
Xiaoru WU
;
Desheng XU
;
Yi FENG
;
Fang GU
- Publication Type:Journal Article
- Keywords:
Fibre Ophiopogon;
saponins;
macroporous resin;
ELSD
- From:
Chinese Traditional Patent Medicine
1992;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a HPLC-ELSD method for determining ophiopogonin D and ophiopogonin D′ in Fibre Ophiopogon enriched extract by macroporous resin. METHODS: The determination was performed by HPLC-ELSD on APOLLO C_(18) column(100 A,5 ?,4.6 mm?250 mm),using acetonitrile-H_2O(50∶50) as a mobile phase,flow rate was at 1.0 mL/min,column temperature was at 25℃,detector:ELSD(Neu:70℃,Eva:90℃,gas flow:1.5 SLM). RESULTS: The linear range of ophiopogonin D was 4.424-22.12 ?g,r=0.999 7,The average recovery was 97.6%,RSD=1.51%.The linear range of ophiopogonin D′ was 3.848-19.24 ?g,r=0.999 8.The average recovery was 98.4%,RSD=4.8%. CONCLUSION: The method is convenient,sensitive and accurate.
