HPLC Determination of Scutellarin and Chlorogenic Acid in Qingkailing Extract
- VernacularTitle:HPLC法测定清开灵膏中黄芩苷与绿原酸的含量
- Author:
Yanfang XIAN
;
Juan SUO
;
Wen HUANG
;
Ziren SU
- Publication Type:Journal Article
- Keywords:
Qingkailing extract;
Scutellarin;
Chlorogenic acid;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the contents of scutellarin and chlorogenic acid in Qingkailing extract by HPLC.Methods Kromasil C18 column was adopted and the mobile phase consisted of methnol-water-phosphoric acid (20 ∶80 ∶0.2) with the flow rate being 1.0 mL?min-1 and column temperature being 25 ℃.The detection wavelength was 327 nm.Results The linearity of scutellarin was good in the range of 0.090～0.540 ?g,r=0.999 9,and its average recovery rate was 100.3 %(RSD=0.99 %);the linearity of chlorogenic acid was good in the range of 0.196～1.176 ?g,r=0.999 9,and its average recovery rate was 99.9 %(RSD=1.06 %).Conclusion This method is sensitive,simple,accurate,and can be used for the quality control of Qingkailing extract.
