HPLC Determination of Berberine Chloride in Lianpu Shuangqing Tablet
- VernacularTitle:HPLC法测定连蒲双清片中盐酸小檗碱的含量
- Author:
Jianhong LIU
;
Guanglan LI
- Publication Type:Journal Article
- Keywords:
Lianpu Shuangqing Tablet;
HPLC;
Berberine Chloride;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the berberine chloride content in Lianpu Shuangqing Tablet and to develop a steady and reliable method for the quality control of the tablet.Method A HPLC method was adopted.The column was Dionex C18 (5 ?m,4.6 mm ?150 mm)with the column temperature of 28 ℃,the mobile phase was acetonitrile-0.033 mol/L potassium dihydrogen phosphate(25 ∶75) with the flow rate of 1.0 mL/min,and the detection wavelength was at 346 nm.Results Berberine chloride has a linearity in the range of 0.153～1.224 ?g,and the average content in the tablet was 1.26 mg/tablet.Conclusion This method is simple,accurate,and can be used for the quality control of Lianpu Shuangqing Tablet.
