Effect of Yiqi Huoxue Formula on Expression of E-CD and Invasion of Human Lung Cancer Cell Lines in Vitro
- VernacularTitle:益气活血方对肺癌细胞株上皮细胞粘附分子表达及侵袭能力的影响
- Author:
Bingsheng WANG
;
Deguang SHI
;
Shuyue ZHAI
- Publication Type:Journal Article
- Keywords:
epithelial cadherin;
Yiqihuoxuezongyao;
lung cancer cell cultured NC-H446
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Chinese herbal medicine of Yiqi Huoxue (YQHX) on proliferation and expression of E-CD and invasion of human lung cancer cell lines - small cell lung cancer (NC-H446) in vitro. Methods By cell measured method, immunohistochemistry method, technique of image and Boyden Chamber method, the effects of YQHX or all trans-tretinoin acid (ARTA) on proliferation and expression of E-CD and invasion of NC-H446 cell lines with different time cultured by YQHX or ARTA in vitro were studied. Results The proliferation of NC-H446 were inhibited significantly by 5% YQHX or 10-5 mmol/L ARTA (P0.05). The shape and structure of cell NC-H446 was occurred variant. YQHX enhanced remarkably expression of E-CD of NC-H446 with 36 hour cultured (P 0.05). The quantity of NC-H446 cells crossed Matrigel and polyvinylpyrrolidone-free polycarbonate filter were more than the control group (P 0.05). Conclusion YQHX can inhibit the proliferation, enhance expression of E-CD on NC-H446, and decrease invasion and metastasis of NC-H446.
