Content Determination of Ursolic Acid in Shanzhajing Jiangzhi Tablets by HPLC
- VernacularTitle:HPLC法测定山楂精降脂片中熊果酸的含量
- Author:
Jianbao DING
;
Rile GE
;
Jianmin ZHANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Shangzhajing Jiangzhi Tablets;
ursolic acid;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine ursolic acid in Shanzhajing Jiangzhi Tablets by HPLC method. Method Chromatographic column was Kromasil C18 (4.6 mm?200 mm, 5 ?m). The Mobile phase was methanol- 0.15% phosphoric acid (88∶12), and the flow rate was 1.0 mL/min. Detection wavelength was 215 nm. Column temperature was room temperature. Results Ursolic acid quantity and its peak area showed good linearity relationship in the range of 0.253～5.052 ?g. The correlation coefficient (r ) was 0.9998 (n=6). The average recovery was 100.37% and RSD was 1.88%. Conclusion The method is easy and accurate, with good sensitivity and repeatability. It is a good method to control the quality of Shanzhajing Jiangzhi Tablets.
